Healthy soils produce healthy crops

Clubroot testing with maximum reliability

Exova tests for the presence of clubroot spores through the PCR method (Polymerase Chain Reaction). Precisely, we quantify Plasmodiophora brassicae DNA by Real-Time PCR (or qPCR), which has been proven reliable for identifying very low levels of clubroot infection.

Our developed methodology for clubroot testing has added advantages over other methods. It allows:

  • accurate DNA quantification
  • a high degree of specificity
  • faster results than traditional methods of DNA analysis.

Knowing the quantity of spores in soil rather than a simple presence/absence result allows for evaluating field distribution of spore infestation and helps to assess the risk of disease manifestation.

Specificity of detection of the target DNA is accomplished by using custom primers and probes that are based on the DNA sequence which is unique to Plasmodiophora brassicae alone.

Real-time PCR does not require extra steps, like gel-electrophoresis, which allows us to complete rush analysis in as little as 2 days. Our regular turn-around-time is five days.

Clubroot testing to identify the threat of clubroot

Clubroot is a significant threat to the canola industry and a primary concern for commercial producers worldwide, with severe outbreaks causing considerable crop losses, which makes clubroot testing a vital requirement from a business point of view. This soil-borne disease is caused by an unusual organism called Plasmodiophora brassicae. The pathogen is not only particularly hardy, but it also doesn’t easily fit into the standard classification of life, which makes clubroot a challenge to control.

Clubroot testing is a beneficial practice to prevent the spread of clubroot infestation and ensure healthy food production.